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Education
BS; University
of Pittsburgh; 1975
MS; University
of Pittsburgh; 1978
ScD; University
of Pittsburgh; 1983
Research Interests
Real-time PCR for measuring Epstein-Barr Virus DNA load in transplant patients; Streptococcus pyogenes and viruses including rhinovirus as agents of common cold; Infectious Agents of Persistent Cough
Research Summary
Dr. Wadowsky is interested in determining if Streptococcus pyogenes causes a common cold syndrome in children. Streptococcus pyogenes is an important cause of "Strep throat" and accurate detection of this bacterium facilitates prompt treatment and prevention of serious sequellae. Dr. Wadowsky has received funding from the Research Advisory Committee of Children's Hospital of Pittsburgh to develop a multiplex real time PCR assay to detect rhinoviruses in respiratory secretions. This work is expected to lead to the availability of a diagnostic test that can be used to exclude the presence of rhinoviruses in respiratory tract secretions. Dr. Wadowsky is also interested in exploring the application of quantitative real time PCR for monitoring Epstein Barr virus DNA load in blood specimens from transplant patients and the value of this test for diagnosing infectious mononucleosis. He is also interested in studying ways in which to improve the diagnosis of respiratory tract infection caused by fastidious bacteria including Bordetella pertussis, Chlamydia pneumoniae, and Mycoplasma pneumoniae.
Dr. Wadowsky is also Director of Pediatric Molecular Microbiology and Consultant in Diagnostic Microbiology at the Children's Hospital of Pittsburgh. He is responsible for developing molecular assays to improve the diagnosis of infection in children.
Affiliations
Recent Publications
- Castilla, E.A. and Wadowsky, R.M. (2000). Effect of a Mycoplasma hominis-like Mycoplasma on the infection of HEp-2 cells by the TW-183 strain of Chlamydia pneumoniae. J. Clin. Microbiol. 38:861-862.
- Wadowsky, R.M., Castilla, E.A., Laus, S., Kozy, A., Atchison, R.W., Kingsley, L.A., Ward, J.I., Greenberg, D.P. and Adult Pertussis Vaccine Efficacy Study Group. (2002). Evaluation of Chlamydia pneumoniae and Mycoplasma pneumoniae as etiologic agents of persistent cough in adolescents and adults. J. Clin. Microbiol. 40:637-640.
- Pitetti, R.D., Laus, S., and Wadowsky, R.M. (2003). Clinical Evaluation of a quantitative real-time PCR assay for the diagnosis of primary Epstein-Barr virus infection in children. Pediatr. Infect. Dis. J. 22: 736-739.
- Wadowsky, R.M., Laus, S., Green, M., Webber, S.A., and Rowe, D. (2003). Measurement of Epstein-Barr Virus DNA Loads Measured in whole blood and plasma by TaqMan PCR and in peripheral blood lymphocytes by competitive PCR. J. Clin. Microbiol. 41: 5245-5249.
- Shen, K., Antalis, P., Gladitz, J., Sayeed, S., Ahmed, A., Yu, S., Hayes, J., Johnson, S., Dice, B., Dopico, R., Keefe, R., Janto, B., Chong, W., Goodwin, J., Wadowsky, R.M., Erdos, G., Post, J.C., Ehrlich, G.D., and Hu, F.Z. (2005) Identification, distribution, and expression of novel genes in 10 clinical isolates of nontypeable Haemophilus influenzae. Infect. Immun. 73:3479-3491.
Last Updated: October 7, 2007
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